Electroporador de genes GP-3000

Descrição do produto
O electroporador de genes GP-3000 consiste na unidade principal, copo de electroporação de genes e cabos de ligação dedicados. Utiliza principalmente a electroporação para introduzir ADN em células competentes, células vegetais e animais e células de levedura. Em comparação com outros métodos, o electroporador de genes oferece alta repetibilidade, alta eficiência, facilidade de operação e controlo preciso. Além disso, a electroporação não tem genotoxicidade, tornando-a uma técnica essencial em biologia molecular.
Princípio de funcionamento
Cell electroporation (also called cellular electroporation) is an important method for introducing exogenous macromolecules such as DNA, RNA, siRNA, proteins, and small molecules into cells.
Under the influence of a strong, momentary electric field, the cell membrane in the solution becomes permeable, allowing charged exogenous substances to enter the cell membrane in a manner similar to electrophoresis. Due to the high resistance of the cell membrane’s phospholipid bilayer, the voltage produced by the external electric field is borne by the cell membrane, with minimal voltage distributed to the cytoplasm, result- ing in negligible intracellular current and low cytotoxicity during electroporation. After DNA or other substances enter the cell membrane, they remain near the membrane, with subsequent diffusion into the nucleus or other parts facilitated by the cell’s mechanisms.
Since electroporation is a physical method, the molecular properties of the cell surface have little effect on the process. Compared to chemical transfection methods or viral vector transfection, electroporation can be applied to all cell types and is easy to quantify.
Diagrama do campo elétrico da electroporação celular
1 The cell membrane behaves like an insulator, causing current distortion in the electroporation fluid near the cell.
2 In a series circuit, the larger the resistance, the higher the voltage; most of the voltage is borne by the cell membrane at both ends.
3 Apenas uma extremidade da célula é effectivamente electroporada.
4 The voltage distributed to the cytoplasm is very small; DNA stops at the membrane after electro- poration, and its subsequent diffusion is driven by the cell’s natural mechanisms.
5 The voltage at both ends of the nucleus is extremely small, and this small voltage is borne by the nuclear membrane, leaving the interior of the nucleus completely voltage-free, which eliminates genotoxicity during electroporation.

Caraterísticas do produto
High Efficiency                Short transformation time, high conversion rate, excellent repeatability.
Intelligent Storage          Experimental parameters can be stored and easily retrieved by users
Precise Control               Pulse discharge is controlled by a microprocessor.
Aesthetic Design            Integrated design, intuitive display, and easy operation.

Application Areas

· Electroporation of bacteria, yeast, and other microorganisms.
· Transfection of mammalian cells, plant tissues, and protoplasts.
· Cell hybridization and gene fusion.
· Introduction of marker genes for labeling and indicating purposes.
· Introduction of drugs, proteins, antibodies, and other molecules for studying cellular structure and function.

Parâmetros técnicos

Taxas de conversão para diferentes estirpes

Nota: Devido a variações nas condições experimentais em diferentes laboratórios, os parâmetros acima são apenas para referência.

Exemplos de experiências
1、LIJ, ZHANG S, GAO L, et al. A cell-based high-throughput assay for the screening of small-molecule inhibitors of p53-MDM2 interaction [J]. Journal of biomolecular screening, 2011.
2 、 ZHANG SY, LIJ, XIE X. Descoberta e caraterização de novos agonistas de pequenas moléculas do recetor 119 acoplado à proteína G [J]. Ata farmacológica Sinica, 2014.