Electroporador de genes SCIENTZ-2CS

Product  Description
The SCIENTZ-2CS Gene Electroporator consists of the main unit, gene electroporation cup, and dedicated connecting cables. It primarily uses electroporation to introduce DNA into competent cells, plant and animal cells, and yeast cells. Compared to other methods, the gene electroporator offers high repeatability, high efficiency, ease of operation, and precise control. Additionally, electroporation has no genotoxicity, making it an essential technique in molecular biology.
Working   Principle
Electroporation, also known as cellular electroporation, is a key method for introducing exogenous macromolecules like DNA, RNA, siRNA, and proteins, as well as small molecules, into cells.
Under the influence of a transient strong electric field, the cell membrane becomes permeable, allowing charged exogenous substances to enter the cell in a manner similar to electrophoresis. Due to the high resistance of the cell membrane’s phospholipid bilayer, the voltage across the cell is mostly borne by the membrane, with minimal voltage in the cytoplasm. This leads to negligible cytotoxicity during electroporation under normal conditions. Once DNA or other substances pass through the membrane, they stay near the membrane before the cell’s mecha- nisms transport them to the nucleus or other parts of the cell.
Since electroporation relies on physical methods, the molecular characteristics on the cell surface have minimal effect on the process. Unlike chemical transfection or viral vector methods, electroporation can be applied to all cell types and is easily quantifiable.
Electroporación celular Diagrama del campo eléctrico
1 The cell membrane acts as an insulator, causing the electric current in the electroporation fluid near the cell to distort.
2 In a series circuit, the larger the resistance, the higher the voltage; most of the voltage is borne by the cell membrane.
3 Sólo se electropora eficazmente un extremo de la célula.
4 The voltage in the cytoplasm is minimal; DNA halts at the membrane after electroporation and slowly diffuses into the cell via cellular mechanisms.
5 The voltage at the nuclear membrane is extremely low, with no voltage inside the nucleus, resulting in no genotoxicity during electroporation.

Características

Aplicación

• Electroporation of bacteria, yeast, and other microorganisms.
• Transfection of mammalian cells, plant tissues, and protoplasts.
• Cell hybridization and gene fusion.
• Introduction of marker genes for labeling and indicating purposes.
• Introduction of drugs, proteins, antibodies, and other molecules for studying cellular structure and function.

Parámetros técnicos

Índices de conversión para distintas cepas

Nota: Debido a las variaciones en las condiciones experimentales de los distintos laboratorios, los parámetros anteriores son sólo de referencia.

Ejemplos de experimentos
1、 LIJ, ZHANG S, GAO L, et al. A cell-based high-throughput assay for the screening of small-molecule inhibitors of p53- MDM2 interaction [J]. Journal of biomolecular screening, 2011.
2、ZHANG SY, LIJ, XIE X. Discovery and characterization of novel small molecule agonists of G protein-coupled receptor 119 [J]. Acta pharmacologica Sinica, 2014.

1、LIJ and colleagues analyzed the small molecule inhibitors of p53-MDM2 protein  interactions based on high-through-put screening.

2、ZHANG SY and colleagues discovered and identified aunique G-protein-coupled receptor 119 agonist.