{"id":3442,"date":"2025-12-26T02:31:53","date_gmt":"2025-12-26T02:31:53","guid":{"rendered":"https:\/\/www.ansenta.com\/?post_type=product&p=3442"},"modified":"2026-03-07T00:49:07","modified_gmt":"2026-03-07T00:49:07","slug":"gene-electroporator-gp-3000","status":"publish","type":"product","link":"https:\/\/www.ansenta.com\/pt\/produto\/gene-electroporator-gp-3000\/","title":{"rendered":"Electroporador de genes GP-3000"},"content":{"rendered":"

Descri\u00e7\u00e3o do produto<\/b><\/strong>
\nThe GP-3000 Gene Electroporator consists of the main unit, gene electroporation cup, and dedicated connecting cables. It primarily uses electroporation to introduce DNA into competent cells, plant and animal cells, and yeast cells. Compared to other methods, the gene electropora- tor o\ufb00ers high repeatability, high e\ufb03ciency, ease of operation, and precise control. Additionally, electroporation has no genotoxicity, making it an essential technique in molecular biology.
\nWorking Principle<\/b><\/strong>
\nCell electroporation (also called cellular electroporation) is an important method for introducing exogenous macromolecules such as DNA, RNA, siRNA, proteins, and small molecules into cells.
\nUnder the influence of a strong, momentary electric field, the cell membrane in the solution becomes permeable, allowing charged exogenous substances to enter the cell membrane in a manner similar to electrophoresis. Due to the high resistance of the cell membrane\u2019s phospholipid bilayer, the voltage produced by the external electric field is borne by the cell membrane, with minimal voltage distributed to the cytoplasm, result- ing in negligible intracellular current and low cytotoxicity during electroporation. After DNA or other substances enter the cell membrane, they remain near the membrane, with subsequent di\ufb00usion into the nucleus or other parts facilitated by the cell\u2019s mechanisms.
\nSince electroporation is a physical method, the molecular properties of the cell surface have little e\ufb00ect on the process. Compared to chemical transfection methods or viral vector transfection, electroporation can be applied to all cell types and is easy to quantify.
\nCell Electroporation Electric Field Diagram<\/b><\/strong>
\n1 The cell membrane behaves like an insulator, causing current distortion in the electroporation fluid near the cell.
\n2 In a series circuit, the larger the resistance, the higher the voltage; most of the voltage is borne by the cell membrane at both ends.
\n3 Only one end of the cell is e\ufb00ectively electroporated.
\n4 The voltage distributed to the cytoplasm is very small; DNA stops at the membrane after electro- poration, and its subsequent di\ufb00usion is driven by the cell\u2019s natural mechanisms.
\n5 The voltage at both ends of the nucleus is extremely small, and this small voltage is borne by the nuclear membrane, leaving the interior of the nucleus completely voltage-free, which eliminates genotoxicity during electroporation.<\/p>\n

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Carater\u00edsticas do produto<\/strong>
\nHigh E\ufb03ciency\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Short transformation time, high conversion rate, excellent repeatability.
\nIntelligent Storage\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Experimental parameters can be stored and easily retrieved by users
\nPrecise Control\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0Pulse discharge is controlled by a microprocessor.
\nAesthetic Design\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Integrated design, intuitive display, and easy operation.<\/p>\n

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Application Areas<\/strong><\/p>\n

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\u00b7 Electroporation of bacteria, yeast, and other microorganisms.
\n\u00b7 Transfection of mammalian cells, plant tissues, and protoplasts.
\n\u00b7 Cell hybridization and gene fusion.
\n\u00b7 Introduction of marker genes for labeling and indicating purposes.
\n\u00b7 Introduction of drugs, proteins, antibodies, and other molecules for studying cellular structure and function.<\/p>\n

Technical Parameters<\/strong><\/p>\n

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Conversion Rates for Di\ufb00erent Strains<\/strong><\/p>\n

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Note: Due to variations in experimental conditions across di\ufb00erent labs,the above parameters are for reference only.<\/p>\n

Example Experiments<\/strong>
\n1\u3001LIJ, ZHANG S, GAO L, et al. A cell-based high-throughput assay for the screening of small-molecule inhibitors of p53-MDM2 interaction [J]. Journal of biomolecular screening, 2011.
\n2\u3001ZHANG SY, LIJ, XIE X. Discovery and characterization of novel small molecule agonists of G protein-coupled receptor 119 [J]. Acta pharmacologica Sinica, 2014.<\/p>\n

\"\"<\/p>","protected":false},"excerpt":{"rendered":"

Marca:<\/strong><\/span>ANSENTA
\nOrigem do produto:<\/strong><\/span>CHINA
\nPrazo de entrega:<\/strong><\/span>No prazo de 7 dias
\nCapacidade de fornecimento:<\/strong><\/span>Fornecimento direto da f\u00e1brica
\nModelo:<\/strong><\/span>Electroporador de genes GP-3000
\nIntrodu\u00e7\u00e3o:<\/strong><\/span>
\nThe GP-3000 Gene Electroporator consists of the main unit, gene electroporation cup, and dedicated connecting cables. It primarily uses electroporation to introduce DNA into competent cells, plant and animal cells, and yeast cells. Compared to other methods, the gene electropora- tor o\ufb00ers high repeatability, high e\ufb03ciency, ease of operation, and precise control. Additionally, electroporation has no genotoxicity, making it an essential technique in molecular biology.
\nDescarregar:<\/strong><\/span>
\nElectroporador de genes GP-3000<\/a><\/p>","protected":false},"featured_media":3972,"template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Product Origin:CHINA Delivery time:Within 7 days Supply capacity:Factory Direct Supply Model:Gene Electroporator GP-3000 Introduction: The GP-3000 Gene Electroporator consists of the main unit, gene electroporation cup, and dedicated connecting cables. It primarily uses electroporation to introduce DNA into competent cells, plant and animal cells, and yeast cells. Compared to other methods, the gene electropora-…","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.ansenta.com\/pt\/wp-json\/wp\/v2\/product\/3442","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ansenta.com\/pt\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.ansenta.com\/pt\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ansenta.com\/pt\/wp-json\/wp\/v2\/media\/3972"}],"wp:attachment":[{"href":"https:\/\/www.ansenta.com\/pt\/wp-json\/wp\/v2\/media?parent=3442"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.ansenta.com\/pt\/wp-json\/wp\/v2\/product_brand?post=3442"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.ansenta.com\/pt\/wp-json\/wp\/v2\/product_cat?post=3442"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.ansenta.com\/pt\/wp-json\/wp\/v2\/product_tag?post=3442"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}