{"id":3442,"date":"2025-12-26T02:31:53","date_gmt":"2025-12-26T02:31:53","guid":{"rendered":"https:\/\/www.ansenta.com\/?post_type=product&p=3442"},"modified":"2026-03-07T00:49:07","modified_gmt":"2026-03-07T00:49:07","slug":"gene-electroporator-gp-3000","status":"publish","type":"product","link":"https:\/\/www.ansenta.com\/es\/producto\/gene-electroporator-gp-3000\/","title":{"rendered":"Electroporador de genes GP-3000"},"content":{"rendered":"

Descripci\u00f3n del producto<\/b><\/strong>
\nEl electroporador de genes GP-3000 consta de una unidad principal, un vaso de electroporaci\u00f3n de genes y cables de conexi\u00f3n espec\u00edficos. Utiliza principalmente la electroporaci\u00f3n para introducir ADN en c\u00e9lulas competentes, c\u00e9lulas vegetales y animales, y c\u00e9lulas de levadura. Comparado con otros m\u00e9todos, el electroporador de genes ofrece una alta repetibilidad, alta eficiencia, facilidad de operaci\u00f3n y control preciso. Adem\u00e1s, la electroporaci\u00f3n no tiene genotoxicidad, lo que la convierte en una t\u00e9cnica esencial en biolog\u00eda molecular.
\nPrincipio de funcionamiento<\/b><\/strong>
\nLa electroporaci\u00f3n celular (tambi\u00e9n llamada electroporaci\u00f3n celular) es un m\u00e9todo importante para introducir macromol\u00e9culas ex\u00f3genas como ADN, ARN, ARNsi, prote\u00ednas y peque\u00f1as mol\u00e9culas en las c\u00e9lulas.
\nBajo la influencia de un campo el\u00e9ctrico fuerte y moment\u00e1neo, la membrana celular en la soluci\u00f3n se vuelve permeable, permitiendo que sustancias ex\u00f3genas cargadas entren en la membrana celular de forma similar a la electroforesis. Debido a la alta resistencia de la bicapa de fosfol\u00edpidos de la membrana celular, el voltaje producido por el campo el\u00e9ctrico externo es soportado por la membrana celular, con un voltaje m\u00ednimo distribuido al citoplasma, lo que resulta en una corriente intracelular insignificante y una baja citotoxicidad durante la electroporaci\u00f3n. Despu\u00e9s de que el ADN u otras sustancias entren en la membrana celular, permanecen cerca de la membrana, con la subsiguiente di\ufb00usi\u00f3n en el n\u00facleo u otras partes facilitada por los mecanismos de la c\u00e9lula.
\nDado que la electroporaci\u00f3n es un m\u00e9todo f\u00edsico, las propiedades moleculares de la superficie celular apenas influyen en el proceso. En comparaci\u00f3n con los m\u00e9todos de transfecci\u00f3n qu\u00edmica o de transfecci\u00f3n de vectores virales, la electroporaci\u00f3n puede aplicarse a todos los tipos celulares y es f\u00e1cil de cuantificar.
\nElectroporaci\u00f3n celular Diagrama del campo el\u00e9ctrico<\/b><\/strong>
\n1 La membrana celular se comporta como un aislante, provocando una distorsi\u00f3n de la corriente en el fluido de electroporaci\u00f3n pr\u00f3ximo a la c\u00e9lula.
\n2 En un circuito en serie, cuanto mayor es la resistencia, mayor es la tensi\u00f3n; la mayor parte de la tensi\u00f3n es soportada por la membrana celular en ambos extremos.
\n3 S\u00f3lo se electropora eficazmente un extremo de la c\u00e9lula.
\n4 El voltaje distribuido al citoplasma es muy peque\u00f1o; el ADN se detiene en la membrana tras la electroporaci\u00f3n, y su di\ufb00usi\u00f3n posterior es impulsada por los mecanismos naturales de la c\u00e9lula.
\n5 El voltaje en ambos extremos del n\u00facleo es extremadamente peque\u00f1o, y este peque\u00f1o voltaje es soportado por la membrana nuclear, dejando el interior del n\u00facleo completamente libre de voltaje, lo que elimina la genotoxicidad durante la electroporaci\u00f3n.<\/p>\n

\"\"<\/p>\n

Caracter\u00edsticas del producto<\/strong>
\nAlta E\ufb03ciencia Corto tiempo de transformaci\u00f3n, alta tasa de conversi\u00f3n, excelente repetibilidad.
\nAlmacenamiento inteligente Los par\u00e1metros experimentales pueden ser almacenados y recuperados f\u00e1cilmente por los usuarios
\nControl preciso La descarga de pulsos est\u00e1 controlada por un microprocesador.
\nDise\u00f1o est\u00e9tico Dise\u00f1o integrado, pantalla intuitiva y f\u00e1cil manejo.<\/p>\n

\"\"<\/p>\n

\u00c1mbitos de aplicaci\u00f3n<\/strong><\/p>\n

\"\"<\/p>\n

- Electroporaci\u00f3n de bacterias, levaduras y otros microorganismos.
\n- Transfecci\u00f3n de c\u00e9lulas de mam\u00edfero, tejidos vegetales y protoplastos.
\n- Hibridaci\u00f3n celular y fusi\u00f3n de genes.
\n- Introducci\u00f3n de genes marcadores con fines de etiquetado e indicaci\u00f3n.
\n- Introducci\u00f3n de f\u00e1rmacos, prote\u00ednas, anticuerpos y otras mol\u00e9culas para estudiar la estructura y funci\u00f3n celulares.<\/p>\n

Par\u00e1metros t\u00e9cnicos<\/strong><\/p>\n

\"\"<\/p>\n

\u00cdndices de conversi\u00f3n para distintas cepas<\/strong><\/p>\n

\"\"<\/p>\n

Nota: Debido a las variaciones en las condiciones experimentales de los distintos laboratorios, los par\u00e1metros anteriores son s\u00f3lo de referencia.<\/p>\n

Ejemplos de experimentos<\/strong>
\n1\u3001LIJ, ZHANG S, GAO L, et al. A cell-based high-throughput assay for the screening of small-molecule inhibitors of p53-MDM2 interaction [J]. Revista de cribado biomolecular, 2011.
\n2\u3001ZHANG SY, LIJ, XIE X. Discovery and characterization of novel small molecule agonists of G protein-coupled receptor 119 [J]. Acta pharmacologica Sinica, 2014.<\/p>\n

\"\"<\/p>","protected":false},"excerpt":{"rendered":"

Marca:<\/strong><\/span>ANSENTA
\nOrigen del producto:<\/strong><\/span>CHINA
\nPlazo de entrega:<\/strong><\/span>En 7 d\u00edas
\nCapacidad de suministro:<\/strong><\/span>Suministro directo de f\u00e1brica
\nModelo:<\/strong><\/span>Electroporador de genes GP-3000
\nIntroducci\u00f3n:<\/strong><\/span>
\nEl electroporador de genes GP-3000 consta de una unidad principal, un vaso de electroporaci\u00f3n de genes y cables de conexi\u00f3n espec\u00edficos. Utiliza principalmente la electroporaci\u00f3n para introducir ADN en c\u00e9lulas competentes, c\u00e9lulas vegetales y animales, y c\u00e9lulas de levadura. Comparado con otros m\u00e9todos, el electroporador de genes ofrece una alta repetibilidad, alta eficiencia, facilidad de operaci\u00f3n y control preciso. Adem\u00e1s, la electroporaci\u00f3n no tiene genotoxicidad, lo que la convierte en una t\u00e9cnica esencial en biolog\u00eda molecular.
\nDescargar:<\/strong><\/span>
\nElectroporador de genes GP-3000<\/a><\/p>","protected":false},"featured_media":3972,"template":"","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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Product Origin:CHINA Delivery time:Within 7 days Supply capacity:Factory Direct Supply Model:Gene Electroporator GP-3000 Introduction: The GP-3000 Gene Electroporator consists of the main unit, gene electroporation cup, and dedicated connecting cables. It primarily uses electroporation to introduce DNA into competent cells, plant and animal cells, and yeast cells. Compared to other methods, the gene electropora-…","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.ansenta.com\/es\/wp-json\/wp\/v2\/product\/3442","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ansenta.com\/es\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.ansenta.com\/es\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ansenta.com\/es\/wp-json\/wp\/v2\/media\/3972"}],"wp:attachment":[{"href":"https:\/\/www.ansenta.com\/es\/wp-json\/wp\/v2\/media?parent=3442"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.ansenta.com\/es\/wp-json\/wp\/v2\/product_brand?post=3442"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.ansenta.com\/es\/wp-json\/wp\/v2\/product_cat?post=3442"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.ansenta.com\/es\/wp-json\/wp\/v2\/product_tag?post=3442"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}